Cancer Detection and Treatment Using Atomic Force Microscopy (AFM)

UC Case No. 2006-707

SUMMARY:

UCLA Researchers in the Department of Chemistry and Biochemistry have applied atomic force microscopy (AFM) to accurately detect tumor cells based on their nanomechanical properties.

BACKGROUND:

Currently tumor cells are detected by histomorphological analysis using stains and antibodies to highlight morphological changes and expression patterns of specific proteins. These histological analyses are typically performed in vitro on fixed tissue samples taken from a patient by invasive procedures (i.e., biopsy). Although the analysis may be simple, it often suffers from low sensitivity or specificity, and does not provide adequate functional measurements concerning tumor cell behavior.

INNOVATION:

AFM is a high-resolution imaging technology that is capable of measuring cell stiffness and cell-surface adhesion of individual living cells. UCLA researchers have used AFM to detect changes in the local nanomechanics inherent to both normal and tumor cells in vitro. Analysis yielded a distinction between tumor and normal cells, and aggressive (invasive, metastatic) and non-aggressive tumors.

POTENTIAL APPLICATIONS:

• Routine diagnostic platform for the detection of cancer cells and analyzing their behavior
• Studies on drug efficacy on cancer cell metastasis and invasion

ADVANTAGES:

• Detection of tumor cells based on physical properties rather than qualitative features
• High accuracy

RELATED MATERIALS:

• Cross, S.E., Jin, Y-S., Rao, J., and Gimzewski, J.K. Nanomechanical analysis of cells from cancer patients. Nature Nanotechnology 2, 780-783 (2007) https://www.nature.com/articles/nnano.2007.388

DEVELOPMENT-TO-DATE:

The invention has been partially tested. A blind study of a sample believed to contain both normal and tumor cells by histomorphological analysis was determined by AFM to contain tumor cells exclusively, despite morphological differences. Deeper histology tests confirmed the accuracy of the AFM results. In addition to detecting tumor cells in vitro, the technology is being explored for diagnostic and therapeutic use in vivo as well.