Antibody-Free Protocol For Generation Of Highly Expandable, Non-Fibroadipogenic Mesodermal Precursors from Human Pluripotent Stem Cells For Treatment
Tech ID: 30170 / UC Case 2018-465-0
SUMMARY
UCLA researchers in the Department of Orthopaedic Surgery have developed a novel method to generate mesodermal precursors from human pluripotent stem cells to treat chronic skeletal muscle atrophy and fibrosis.
BACKGROUND
Cell based therapy has shown great promise in treating human muscle atrophy and fibrosis. Mesodermal progenitor cells provide a plentiful, precisely identified cell source that can be reproducibly induced to produce desired therapeutic cells. However, there is a shortage of suitable cell sources for the derivation of mesodermal progenitor cells, and cell sources greatly impact the differentiation and therapeutic efficiency of the drive progenitor cells. There is an unmet need to derive mesodermal progenitor cells of high quality and sufficient quantity.
INNOVATION
UCLA researchers in the Department of Orthopaedic Surgery have developed a novel method to derive mesodermal cells that will be sufficient for effective treatment of human muscle injury. A single step protocol is used to for generation of mesodermal cells from human pluripotent stem cells with 90% efficiency. Expansion of the derived cells resulted in a 104-fold increase in cell number within few weeks, without any additional cell sorting or isolation. When the derived mesodermal stem cells were injected in a murine muscle injury model, the numbers of diseased atrophic muscle myofibers significantly decreased.
APPLICATIONS
Stem cell therapy
Progenitor cell derivation
Off-the-shelf cell therapy
Cell-based drug screening
Fibroadipogenic diseases of muscle, liver, lung, kidney, bone marrow and heart
ADVANTAGES
Cost-effective, omits the use of clinical grade antibodies or enzymes
Cell source (human embryonic stem cells) plentiful
Cell derivatives are phenotypically stable and uniform
Cell derivatives are highly stable for cryopreservation
Cell derivatives are unable to differentiate into adipocytes or fibroblasts?
RELATED MATERIALS
Devana SK, Kelley BV, McBride OJ, Kabir N, Jensen AR, Park SJ, Eliasberg CD, Dar A, Mosich GM, Kowalski TJ, Péault B, Petrigliano FA, SooHoo NF. Adipose-derived Human Perivascular Stem Cells May Improve Achilles Tendon Healing in Rats. Clin Orthop Relat Res. 2018 Oct;476(10):2091-2100.
Jensen AR, Kelley BV, Eliasberg C, Devana SK, Murray IR, McAllister DR, Péault B, Dar A, Petrigliano FA. PDGFRa+PDGFRß+ Fibroadipogenic progenitor cells contribute to fatty infiltration and fibrosis following massive rotator cuff tears in a murine model. J Shoulder Elbow Surg. 2018 Jul;27(7):1149-1161.
PATENT STATUS
Patent Pending