UCLA researchers from the Department in Neurology have developed a spatiotemporal illumination microscope system. The system includes a multi-well plate configured to receive live cells and a DMD (Digital Micromirror Device) configured to deliver targeted illumination to selectively illuminate a plurality of the wells. The system further includes a tandem-lens system having an objective lens and an imaging lens positioned facing each other to capture fluorescent light emitted from the cells. The system also includes an image sensor that receives fluorescent light captured by the tandem-lens system, generating imaging data. The system further includes a controller configured to use the imaging data to control closed-loop modulation of the live cells.
KEYWORDS: Fluorescence microscopy