Summary:
UCLA researchers in the Department of Mechanical and Aerospace Engineering have developed a novel label-free optical mapping method for profiling real-time biomechanical dynamics over larger areas with high spatiotemporal resolution.
Background:
Mapping biomechanical dynamics often involves the use of various advanced technologies to measure and analyze mechanical properties. Current methods include traction force microscopy, which measures movements and forces within biological systems. This technique can provide valuable insight into the functions of biological systems for improving clinical diagnostics and enhancing development of drug therapy. Currently, these technologies for mapping large-area biomechanical properties are still limited, being restricted by a small field of view and scanning capabilities. In addition, these methods may require the use of a fluorescent dye for labelling cells, which risks interfering with the physiological activities of cells. Therefore, there is an unmet need for a new method which resolves these imaging problems and enhances spatiotemporal resolution of biological samples.
Innovation:
UCLA researchers in the Department of Mechanical and Aerospace Engineering have invented a novel label-free optical mapping method, with observation field of view up to 10.6 mm2. This new field of view is three orders of magnitude better than conventional methods such as traction force microscopy or micropillar arrays. High spatiotemporal resolution is maintained to enable measurement of transient activities at the cellular level. The inventors successfully demonstrated this innovation by measuring the forces generated from neonatal rat ventricular myocytes (NRVMs). These features are promising for studying the complex interplay between biomechanical, biochemical and bioelectric properties within biological systems. This platform can be integrated with a fluorescent imaging system to conduct simultaneous mappings of the aforementioned properties.
Potential Applications:
- Cell imaging
- Cell differentiation
- Biomedical Research
- Integration with fluorescent imaging systems
Advantages:
- Label-free
- High spatiotemporal resolution
- Observation field of view up to 10.6 mm2
- Real-time monitoring of complex biological systems
Development-To-Date:
First successful demonstration of prototype in June 2023
Publication Pre-Print:
Label-Free Optical Mapping for Large-Area Biomechanical Dynamics of Multicellular Systems
Reference:
UCLA Case No. 2023-290
Lead Inventor:
Pei-Yu Chiou